Basic Information and Protocols (PDF)

• Flow Cytometry Glossary
• Minimizing Background Staining
• Blocking FC-Receptor Binding
• Monoclonal Antibody Staining Procedure
• Staining Procedure for Whole Blood
• Preparation of 2% Formaldehyde Solution
• DNA Staining for Cell Cycle Analysis
• Cell Sorting Q and A
• Cell Surface Staining for Sorting
• Cell Sorting Tips ‘n’ Tricks

Specialty Protocols (PDF)

• Propidium Iodide Staining of Dead Cells
• 7-AAD Staining of Dead Cells
• Method for EtOH fixation of cells for long-term storage and DNA staining
• Intracellular Antigen Staining
• Surface Antigen Staining and DNA Content
• Simultaneous Measurement of Apoptosis and Necrosis
• Detection of Apoptosis by 7-AAD Staining
• CD 38
  • CD38 Fluorescence Intensity Quantitation
  • CD38 Fluorescence Intensity Quantitation Data (Excel Document)
  • References (Word Document)
• Measurement of GFP Expression and DNA Content in Permeabilized Cells
• Measurement of GFP Expression and DNA Content in Unfixed Cells
• Measurement of Cell Surface Immunofluorescence, Viability, and DNA content
• Measurement of Immunofluorescence and DNA/RNA Content
• Measurement of Intracellular CA2+ and Cell Surface Antigen Expression