Lab Protocols
Basic Information and Protocols
- Flow Cytometry Glossary
- Minimizing Background Staining
- Blocking FC-Receptor Binding
- Monoclonal Antibody Staining Procedure
- Staining Procedure for Whole Blood
- Preparation of 2% Formaldehyde Solution
- DNA Staining for Cell Cycle Analysis
- Cell Sorting Q and A
- Cell Surface Staining for Sorting
- Cell Sorting Tips ‘n’ Tricks
Specialty Protocols (PDF)
All PDFs below are available in Box. Please contact us shaile@mednet.ucla.edu if you have any questions or concerns.
- Propidium Iodide Staining of Dead Cells
- 7-AAD Staining of Dead Cells
- Method for EtOH fixation of cells for long-term storage and DNA staining
- Intracellular Antigen Staining
- Surface Antigen Staining and DNA Content
- Simultaneous Measurement of Apoptosis and Necrosis
- Detection of Apoptosis by 7-AAD Stainin
- CD38 Fluorescence Intensity Quantitation
- Measurement of GFP Expression and DNA Content in Permeabilized Cells
- Measurement of GFP Expression and DNA Content in Unfixed Cells
- Measurement of Cell Surface Immunofluorescence, Viability, and DNA content
- Measurement of Immunofluorescence and DNA/RNA Content
- Measurement of Intracellular CA2+ and Cell Surface Antigen Expression